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Identification and evaluation of new reference genes inGossypium hirsutumfor accurate normalization of real-time quantitative RT-PCR data

✍ Scribed by Sinara Artico; Sarah M Nardeli; Osmundo Brilhante; Maria Fátima Grossi-de-Sa; Marcio Alves-Ferreira


Book ID
115007815
Publisher
BioMed Central
Year
2010
Tongue
English
Weight
564 KB
Volume
10
Category
Article
ISSN
1471-2229

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## Abstract Careful validation of reference genes used for the normalization of real‐time RT‐PCR data is required to obtain accurate results regarding gene expression. We evaluated the stability of seven commonly used reference genes in the cerebral cortex and hippocampus of rats 3 days following t