## Abstract Careful validation of reference genes used for the normalization of realβtime RTβPCR data is required to obtain accurate results regarding gene expression. We evaluated the stability of seven commonly used reference genes in the cerebral cortex and hippocampus of rats 3 days following t
β¦ LIBER β¦
Validation of Candidate Reference Genes for the Accurate Normalization of Real-Time Quantitative RT-PCR Data in Rice During Seed Development
β Scribed by Qian-Feng Li; Samuel S. M. Sun; Ding-Yang Yuan; Heng-Xiu Yu; Ming-Hong Gu; Qiao-Quan Liu
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 429 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0735-9640
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## Background An essential part of using real-time RT-PCR is that expression results have to be normalized before any conclusions can be drawn. This can be done by using one or multiple, validated reference genes, depending on the desired accuracy of the results. In the pig however, very little inf