✦ LIBER ✦

Ice nucleation temperature influences recovery of activity of a model protein after freeze drying

✍ Scribed by Teresa Cochran; Steven L. Nail

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 97 KB
Volume: 98
Category: Article
ISSN: 0022-3549
DOI: 10.1002/jps.21815

No coin nor oath required. For personal study only.

✦ Synopsis

The objective of this study was to determine whether a relationship exists between ice nucleation temperature and recovery of activity of a model protein, lactate dehydrogenase, after freeze drying. Aqueous buffer systems containing 50 microg/mL of protein were frozen in vials with externally mounted thermocouples on the shelf of a freeze dryer, then freeze dried. Various methods were used to establish a wide range of ice nucleation temperatures. An inverse relationship was found between the extent of supercooling during freezing and recovery of activity in the reconstituted solution. The data are consistent with a mechanism of inactivation resulting from adsorption of protein at the ice/freeze-concentrate interface during the freezing process.

📜 SIMILAR VOLUMES

Modeling of freezing step during vial fr

Modeling of freezing step during vial freeze-drying of pharmaceuticals—influence of nucleation temperature on primary drying rate

✍ Kyuya Nakagawa; Aurelie Hottot; Severine Vessot; Julien Andrieu 📂 Article 📅 2011 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 124 KB

A kinetic model describing cell growth a

A kinetic model describing cell growth and production of highly active, recombinant ice nucleation protein in Escherichia coli

✍ M. A. Palaiomylitou; K. A. Matis; A. I. Zouboulis; D. A. Kyriakidis 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 205 KB 👁 2 views