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Hysteretic kinetic behavior of beef liver pyruvate carboxylase

✍ Scribed by Berwin P. Yip; Frederick B. Rudolph


Book ID
115703839
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
854 KB
Volume
191
Category
Article
ISSN
0003-9861

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A technique to measure the activity of pyruvate carboxylase spectrophotometritally in crude liver homogenates is described. The assay is based on the transformation of oxaloacetate, which is formed during the carboxylation reaction, into citrate in the presence ofexcess acetyl CoA and citrate syntha