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Kinetic Characterization of Yeast Pyruvate Carboxylase Isozyme Pyc1 †

✍ Scribed by Branson, Joy P.; Nezic, Mark; Wallace, John C.; Attwood, Paul V.


Book ID
121314495
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
84 KB
Volume
41
Category
Article
ISSN
0006-2960

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We have cloned and characterized a gene encoding pyruvate carboxylase from the methylotrophic yeast Pichia pastoris. Disruption of this gene produced inability to grow in minimal medium with glucose as carbon source and ammonium as nitrogen source. Growth was possible with aspartate or glutamate as