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Hyperresistance to DNA damaging agents in yeast

✍ Scribed by Axel Ruhland; Martin Brendel; Robert H. Haynes

Book ID
104768794
Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
474 KB
Volume
11
Category
Article
ISSN
0172-8083

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✦ Synopsis

In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxide, formaldehyde, and alkylating agents were isolated and the respective hyperresistance determinants shown to co-segregate with the vector plasmid. Phenotypical characterization indicated different degrees of resistance, few cases of cross-resistance and differing structural stability of the cloned DNA. By transfer to E. coli and subsequent retransformation of yeast a number of plasmids was shown to stably carry the genetic information for hyperresistance.

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## Abstract The __Saccharomyces cerevisiae__ SPT10 protein possesses a DNA‐binding domain that is fused to a putative histone acetyltransferase domain. It binds specifically to upstream‐activating sequence elements in the core histone promoters and plays a direct role in histone gene regulation. SP