✍ Scribed by Andrea B. Schmid; Per Flodin
No coin nor oath required. For personal study only.
Porous particles were made by suspension copolymerization of trimethylolpropane trimethacrylate with N,N'-methylene-bis-acrylamide using ethyl acetate as pore-forming agent. The concentration of N,N'-methylene-bis-acrylamide was varied. The polymers were characterized by scanning electron microscopy, laser diffraction for particle size determination, mercury porosimetry, nitrogen adsorption desorption technique and elemental analysis for nitrogen. The porosity in the swollen state was evaluated by size exclusion chromatography. The analysed amount of nitrogen showed that 82% or more of the N,N'-methylene-bis-acrylamide had polymerized. The pore size distribution became narrower as the amount of N,N'-methylene-bis-acrylamide in the gels increased. In columns packed with water swollen gels, polysaccharide standards were eluted at varying volumes making it possible to construct a calibration curve and to calculate pore size distributions in the wet state. No separation was however obtained with standard proteins. NOMENCLATURE
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