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Hydrolysis of Synthetic and Natural Triglycerides by an Intracellular Lipase from Aspergillus niger

✍ Scribed by Tahoun, M. K. ;El-Kadey, M. F. ;Wahba, A. A.

Publisher: John Wiley and Sons
Year: 1987
Weight: 306 KB
Volume: 89
Category: Article
ISSN: 0931-5985
DOI: 10.1002/lipi.19870890704

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✦ Synopsis

An intracellular lipase was isolated from Aspergillus niger and the enzyme was purified by gel filtration chromatography on Sephadex G 75 column.

The purified enzyme was found to be homogeneousasjudged by electrophoresison polyacryl amidegels.The specificity ofthelipase towardssingle fatty acid triacylglycerols and natural triglycerides was determined. The enzyme preferentially attacks tripalmitoylglycerol as compared to other triglycerides. Positional specificity on using milk fat and olive oil triglycerides showed that the enzyme attacks positions 1 and 3 in triglyceride molecules.

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