Hydrolysis of inulin: A kinetic study of the reaction catalyzed by an inulinase from Aspergillus ficuum

✍ Scribed by Paolo Carniti; Pier Luigi Beltrame; Donatella Guardione; Bonaventura Focher; Annamaria Marzetti

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 444 KB
• Volume: 37
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260370612

✦ Synopsis

A kinetic study of the hydrolysis of inulin was performed by using as catalyst a commercial inulinase from Aspergillus ficuum. The reaction was studied carrying out initial rate as well as time course measurements. Both inulinase and in- vertase activities of the enzyme were taken into account, and the corresponding kinetic parameters were determined in the temperature range 30-50°C. The activation energies of the turnover constant for inulinase and invertase activities were found to be similar (56-57 kJ . mol-'). The ratio S// of invertase to inulinase activity was 1.6 regardless of temperature. The thermal degradation of the enzyme was also investigated up to 70T, and an activation energy of 350-370 k J mol-' was evaluated.