✍ Scribed by G. Kuncová; Y. Maléterová; P. Lovecká
No coin nor oath required. For personal study only.
Various inorganic oxide materials coated with 3-aminopropyltriethoxysilane and glutaraldehyde were examined as support materials for lipase catalysing the hydrolysis of rapeseed oil. Nine commercial glass products of different structure and surface area and magnetite were compared. Hydrolytical activity of channel-like carriers was 100x higher than that of controlled pore glass, glass cloth and powders. Lipase on magnetite was tested in hydrolysis of oil in industrial waste having viscosity of 30mPa.s. After 100% conversion of oil the lipase on magnetite was separated in magnetic field and reused 7x without decrease of activity.
📜 SIMILAR VOLUMES
Lipase (EC 3.1.1.3) from Candida rugosa was immobilized with DEAE-Sephadex A50, Sephadex G50, Sephadex LH-20, Amberlite IRA94, and Amberlite XAD-7. The enzyme immobilized with DEAE-Sephadex A50 was found to be most effective for continuous hydrolysis of olive oil in isooctane. For the continuous rea
Lipase from Pseudornonas fluorescens biotype I was immobilized by adsorption of anion exchange resin using glutaraldehyde to enhance the adsorption. The activity yield of the immobilized lipase was very low (below 1%) when lipase activity was measured using emulsion substrate. The activity yield was
The immobilization of lipase from Candida lipolytica on alumina by adsorption for the hydrolysis of ricebran oil is described. Some of the factors which influence the activity of immobilizate and the stability of immobilizate are discussed. About 69% of the initially added enzyme activity is found i