Continuous hydrolysis of oil by immobilized lipase in a countercurrent reactor

✍ Scribed by Yoshitsugu Kosugi; Hideoki Tanaka; Noboru Tomizuka

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 544 KB
• Volume: 36
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260360609

✦ Synopsis

Lipase from Pseudornonas fluorescens biotype I was immobilized by adsorption of anion exchange resin using glutaraldehyde to enhance the adsorption. The activity yield of the immobilized lipase was very low (below 1%) when lipase activity was measured using emulsion substrate. The activity yield was 10-70% when lipase activity was measured using nonemulsion substrate. Countercurrent reactors for hydrolysis of oil using nonemulsion substrate were studied. A fluidized bed reactor was found to be superior to a fixed bed one since in a fixed bed reactor the separation rate of the two layers was slow and the flow rate of the reactor had to be slower than the separation rate. A fluidized bed reactor system equipped with settling compartments and stirring compartments was devised. Continuous lipolysis at 60°C and continuous separation of oily product and water soluble product were performed. After continuous operation for more than 3 months, 70% of the initial activity of the immobilized lipase was observed at the end of the reaction.