✍ Scribed by M. D. Romero; J. Aguado; L. Rodriguez; J. A. Calles
No coin nor oath required. For personal study only.
The kinetics of cellobiose hydrolysis was studied using Pglucosidase from Prnicilliumfuniculosum, both free and immobilized on nylon powder, at different temperatures, pH values, enzymatic activities and initial cellobiose and glucose concentrations. The experimental results were fitted to a kinetic model by considering the substrate and product inhibitions as well as the thermal deactivation of Pglucosidase with a mean deviation of less than 10%. The immobilization of Pglucosidase led to an increase in the stability of the enzyme against changes in the pH value.
📜 SIMILAR VOLUMES
Removal of p-glucosidase (BG) from cellulase is essential to the enzymatic production of cellobiose from cellulose because of the high reactivity of BG with cellobiose to form glucose. Chitosan is a reversibly soluble-insoluble polymer depending on pH, and it does not conjugate with BG in the soluti