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Production of cellobiose by enzymatic hydrolysis: Removal of β-glucosidase from cellulase by affinity precipitation using chitosan

✍ Scribed by Taira Homma; Michihiro Fujii; Jun-Ichi Mori; Tohru Kawakami; Kenji Kuroda; Masayuki Taniguchi


Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
45 KB
Volume
42
Category
Article
ISSN
0006-3592

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Production of cellobiose by enzymatic hy
✍ Taira Homma; Michihiro Fujii; Jun-ichi Mori; Tohru Kawakami; Kenji Kuroda; Masay 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 549 KB

Removal of p-glucosidase (BG) from cellulase is essential to the enzymatic production of cellobiose from cellulose because of the high reactivity of BG with cellobiose to form glucose. Chitosan is a reversibly soluble-insoluble polymer depending on pH, and it does not conjugate with BG in the soluti