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Hydrolysis of carboxymethylcellulose with mixtures of cellulase and β-1,4-glucosidase

✍ Scribed by V Bravo; M P Páez; M Aoulad El-Hadj; A Reyes; A I García

Publisher
Wiley (John Wiley & Sons)
Year
2001
Tongue
English
Weight
135 KB
Volume
77
Category
Article
ISSN
0268-2575

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✦ Synopsis

Abstract

We studied the hydrolysis of carboxymethylcellulose at 50 °C and pH 4.9 with a commercial preparation of cellulase (Celluclast) supplemented with a commercial β‐1,4‐glucosidase product (Novozym). The initial concentration of carboxymethylcellulose was varied between 2 and 12.5 kg m^−3^ for assays with Celluclast and two Novozym/Celluclast ratios (0.5 and 1). We determined the conversion to glucose and the overall conversion in terms of glucose equivalent. Conversion to glucose turned out to be directly proportional to the product of experimental time multiplied by the concentration of Celluclast and the constant of proportionality increased concomitantly with the concentration of Novozym. Overall conversion remained unaffected by the concentration of Novozym.

© 2001 Society of Chemical Industry

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