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Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications

✍ Scribed by Wei, Hui; Mo, Jingjie; Tao, Li; Russell, Reb J.; Tymiak, Adrienne A.; Chen, Guodong; Iacob, Roxana E.; Engen, John R.


Book ID
121290857
Publisher
Elsevier Science
Year
2014
Tongue
English
Weight
828 KB
Volume
19
Category
Article
ISSN
1359-6446

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The rates at which hydrogens located at peptide amide linkages in proteins undergo isotopic exchange when a protein is exposed to depend on whether these amide hydrogens are hydrogen bonded and whether they are D 2 O accessible to the aqueous solvent. Hence, amide hydrogen exchange rates are a sensi