๐”– Bobbio Scriptorium
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Hybridization ofSaccharomyces cerevisiaewithCandida utilisthrough protoplast fusion

โœ Scribed by Celso Perez; Carlos Vallin; Jorge Benitez

Publisher
Springer-Verlag
Year
1984
Tongue
English
Weight
467 KB
Volume
8
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

Auxotrophic mutants of Saccharomyces cerevisiae and Candida utilis were hybridized through protoplast fusion. Spontaneous, UV- and FPA-induced mitotic segregation indicated that after cell fusion, exclusion of the S. cerevisiae nucleus or nuclear fusion followed by preferential loss of S. cerevisiae chromosomes can take place. Some of the hybrids were stable. One of them, expressed mating and sporulation functions of the S. cerevisiae parent. Thus, markers from both parents could be recovered as mitotic and meiotic segregants.

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