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Hybridization of the Bridged Oligonucleotides with DNA: Thermodynamic and Kinetic Studies

✍ Scribed by Pyshnyi, Dmitrii V.; Lomzov, Alexander A.; Pyshnaya, Inna A.; Ivanova, Eugenia M.


Book ID
121476014
Publisher
Adenine Press
Year
2006
Tongue
English
Weight
802 KB
Volume
23
Category
Article
ISSN
0739-1102

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The rates of duplex formation for two octamers of DNA (5β€² d-CACGGCTC/5β€² d-GAGCCGTG and 5β€² d-CACAGCAC/5β€² d-GTGCTGTG), the homologous RNA, and both sets of hybrids in 1 M NaCl buffer have been measured using stopped-flow spectroscopy. In addition, the thermodynamic parameters, Ξ”HΒ°and Ξ”SΒ°, have been de