Hybridization method for quantitation of
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Unni Spaeren; Waldemar Waldeck; Rolf Eliasson
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Article
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1979
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Elsevier Science
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English
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Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975,J. Mol. Biol. 98,503-517.) The filters were then used in hybrid