A potentiometric enzyme biosensor for the direct measurement of organophosphate pesticides was developed. The basic element of this enzyme biosensor was a pH electrode modified with an immobilized organophosphorus hydrolase layer formed by cross-linking OPH with bovine serum albumin and glutaraldehy
Hybrid tissue/enzyme biosensor for pectin
β Scribed by Hideki Horie; Garry A. Rechnitz
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 393 KB
- Volume
- 306
- Category
- Article
- ISSN
- 0003-2670
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β¦ Synopsis
An amperometric biosensor for the determination of pectin was constructed by combining orange peel tissue as a pectinesterase source with a carbon paste alcohol electrode using alcohol oxidase. A slice of orange peel attached to the surface of the alcohol electrode deesterified pectin, the methanol generated by the reaction was monitored by the electrode. A linear response to pectin was observed in the concentration range of 0.1 to 0.9 g I-', No interference from other carbohydrates was observed.
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The development and experimental verification of a theoretical model for immobilized enzyme inhibition biosensors is reported. The model predicts that the percentage of inhibited enzyme (I %), after exposure to an inhibitor, is linearly related to both the inhibitor concentration ([I] B ) and the sq
Coupling of a slice of the mesocsrp of squash (Cucurbita pepo) or cucumber (Cucumis sativus) to a Clark-type oxygen electrode allows 0.02-0.57 mmol 1-l L-ascorbic acid to be determined amperometrically. The method is based on monitoring the decrease in the current of oxygen at an applied potential o