Hybrid tandem mass spectrometry of electrospray-produced peptide ions

✍ Scribed by Kenneth B. Tomer; Steven R. McGown; Leesa J. Deterding

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 630 KB
• Volume: 28
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/oms.1210280511

✦ Synopsis

The collision-induced decomposition spectra of electrospray-produced molecule ions were investigated on a hybrid tandem mass spectrometer. The molecular mass of the peptides investigated ranged from m/z 409 (as the singly protonated molcule ion) to 1758 (as the singly, doubly and triply protonated molecules). The effect of changing the center-of-mass collision energy by changing either the laboratory collision energy or the mass of the target gas was investigated. The spectra of all ions investigated showed a large number of sequence ions representative of the peptides' structures.

4.5 u (50% valley). Sufficient collision gas was used to decrease the abundance of the parent ion by 50% at 145 eV collision energy.

A11 tandem magnetic sector spectra were acquired on a VG ZAB 4F instrument of BE-EB geometry (VG Analytical/Fisons, Altrincham, Manchester, UK). All