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Discrimination of cyclic peptide diastereomers by electrospray ionization tandem mass spectrometry

✍ Scribed by Bo Zheng; Yiquan Liu; Huihui Li; Yunhua Ye; Xingming Gao; Gu Yuan

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
125 KB
Volume
44
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

In this research, the characteristic ions' abundance ratio between two isomers A and B in MS/MS mass spectra was defined as a parameter for discriminating diastereomers. Through this ratio, the discrimination of four pairs of cyclic peptide (CP) diastereomers was successfully achieved. Furthermore, in the analysis of diastereomers' mixtures, both calibration curve and calculational methods were substantiated to have high precision and accuracy. The average absolute errors of the two methods were 2.0 and 2.5% in the 48 measurements of 16 samples, respectively. This research provided a promising approach for the analysis of the CP diastereomers in the fields of asymmetrical synthesis, chiral natural products and structural biology by ESI‐MS/MS. Copyright Β© 2009 John Wiley & Sons, Ltd.

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