Hybrid Nanogels with Physical and Chemical Cross-Linking Structures as Nanocarriers

✍ Scribed by Nobuyuki Morimoto; Takao Endo; Michiko Ohtomi; Yasuhiko Iwasaki; Kazunari Akiyoshi

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 197 KB
• Volume: 5
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200500051

✦ Synopsis

Abstract

Summary: Polymerizable nanogels were prepared by self‐assembly of cholesteryl group‐bearing pullulan (CHP) with methacryloyl groups (CHPMA). The CHPMA nanogel was polymerized with 2‐methacryloyloxyethyl phosphorylcholine (MPC) by radical polymerization in dilute aqueous solution. The solution properties of the polymers in water were investigated by TEM, SEC‐MALS, and fluorescence quenching technique. Monodispersed hybrid nanogels of CHPMA‐MPC (CM nanogels) (25–30 nm in radius of gyration) were obtained by using CHPMA nanogel as a seed‐nanogel. CM nanogels have a dual cross‐linking structure that is physically cross‐linked with the cholesteryl groups and chemically cross‐linked with the MPC polymer chains. CM nanogels trap heat‐denatured carbonic anhydrase B (CAB) and prevent their aggregations. The nanogels maintained the ability of trapping and releasing enzymes by host‐guest interaction of cholesteryl group and cyclodextrin.

Schematic illustration of hybrid nanogel preparation.

magnified imageSchematic illustration of hybrid nanogel preparation.