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Human T cell clones specific for tetanus toxoid: Characterization of antigen specificity and HLA restriction

✍ Scribed by Christian Schmitt; Jean-Jacques Ballet; Monique Agrapart; Bernard Bizzini

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
616 KB
Volume
12
Category
Article
ISSN
0014-2980

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Peripheral blood mononuclear cells (PBMC) from an HLA DRw6/7 individual were stimulated with tetanus toxoid (TT). T cell blasts were cloned by the limiting dilution technique in the presence of TT and irradiated autologous PBMC (iPBMC). Twelve were propagated under interleukin 2 and restimulated wee

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Human T lymphocyte clones (TLC) specific for diphtheria toxoid (DT) were isolated from a DR6/7 individual by cloning in soft agar in vivo sensitized T lymphocytes. We report here the isolation and characterization of 3 of these clones by studying: (a) the kinetic of activation, (b) the surface pheno