Antigen-specific proliferative human T cell clones with specificity for diphtheria toxoid: genetic and molecular restriction by class II antigens

✍ Scribed by Frédéric Triebel; Véronique Missenard-Leblond; Brigitte Autran; Marie-Claude Couty; Dominique Charron; Patrice Debré

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 530 KB
• Volume: 14
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830140805

✦ Synopsis

Human T lymphocyte clones (TLC) specific for diphtheria toxoid (DT) were isolated from a DR6/7 individual by cloning in soft agar in vivo sensitized T lymphocytes. We report here the isolation and characterization of 3 of these clones by studying: (a) the kinetic of activation, (b) the surface phenotypes, (c) the fine specificity for one of the 2 DT chains and (d) the genetic restriction of the proliferative response by the haplotype DR7. Moreover, blocking studies of the proliferative response to DT by various immunochemically characterized anti-HLA-DR monoclonal antibodies indicate that, on the DR7 molecule, more than one Ia determinant may participate in the clonal DT proliferative response. By using human TLC of a defined specificity and well-characterized anti-DR monoclonal antibodies, such studies may help to define the functional repertoire of Ia molecules in man.