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Human Stress Protein hsp70: Overexpression in E. coli, Purification and Characterization

✍ Scribed by Jindal, Satish; Murray, Peter; Rosenberg, Stanley; Young, Richard A.; Williams, Kevin P.


Book ID
109908558
Publisher
Springer Science and Business Media LLC
Year
1995
Tongue
English
Weight
711 KB
Volume
13
Category
Article
ISSN
0733-222X

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The cDNA for human ciliary neuronotrophic factor (CNTF) has been cloned into an expression vector under the control of the T7 promoter. The BL21 strain of E. coli was transformed with this vector. Human CNTF accounted for about 30% of the total bacterial protein after induction with isopropyl-B-D-th