Human recombinant interleukin-1β- and tumor necrosis factor α-mediated suppression of heparin-like compounds on cultured porcine aortic endothelial cells

✍ Scribed by Kazuyuki Shimada; Toshio Ozawa; Masashi Kobayashi

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 835 KB
• Volume: 144
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041440304

✦ Synopsis

Department of Medicine and Cerratricq, Kochi hlcclic,il School, Nankoku-sh/, Koclii 78? (K S ,T 0 ), arid The Third Ilcpxtment of lnternai hkdrone, Joyarria Mecirca/ and Pharmaccuticai Unibersitv, Tovamd 930-0 1 oZ;1 K 1, I q ~a n Cytokiries are known to tip the balance of the coagulant-anlicoagiilant molecules on the endothelial cell surface toward inlravascular coagulation. Their effects on endothelial cell surface-associated heparin-like compounds have not been examined yet. lncorporahon oi' I'"SJsulfatc into heparan sulfate on cultured porcine aortic endothelial cells was suppressed by human rec:ombinant interleukin-1 p (rlL-1 p) or tumor necrosis factor (Y ( r l N t u ) in a dose-and time-dependent manner with little effect on cell number, protein content, and l-'Hlleucine incorporation of cells. Maximal inhibition was achicvcd by incubation of cells with 100 n g h l of rlL-.lP or 5 nginil of r1.NC-w. for 12-24 hours, resulting in a reduction 01 Ihe synthesis of heparan sulfatr on the cell surface by approxiniately joyu. The dose dependency was consistent with that seen in the stimulation of endothelial cell procoagulant activity by each cytokine. The xrppression o i heparan sulfate synthesis was sustained for at least 48 hour5 after pretrealment of' cells with cytokines and was unchanged after the addition of indornethacin or polyinyxin K . The rate