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Human platelets as a source of HL-A antigens: A study of various solubilization techniques

✍ Scribed by Dautigny, A.; Bernier, I.; Colombani, J.; Jollès, P.


Book ID
123496847
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
358 KB
Volume
57
Category
Article
ISSN
0300-9084

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The preparation and purification of HL-A
✍ R. Voigtmann; G. Uhlenbruck; G. I. Pardoe; K. Rogers 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 577 KB

## Abstract HL‐A‐active protein fragments have been isolated from lymphoid sources (tonsillar tissue, cells from lymphoblastoid cell lines (LCL cells)) and from platelets. The yields and specific activities of products obtained by enzymic chaotropic treatment indicate the superiority of 3 M KCl ext

Evaluation of two sources of soluble HL-
✍ M. A. Pellegrino; S. Ferrone; Anna G. Pellegrino; S. K. Oh; R. A. Reisfeld 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 English ⚖ 640 KB

## Abstract Platelets and human serum have been evaluated as sources for extraction of soluble HL‐A antigens. 3 M KCl was found to efficiently solubilize HL‐A antigens from platelets with a recovery ranging between 50 and 100 %. However, because of the low density of HL‐A determinants on platelets,