✦ LIBER ✦

The preparation and purification of HL-A monospecific antigens using platelets and various lymphoid sources

✍ Scribed by R. Voigtmann; G. Uhlenbruck; G. I. Pardoe; K. Rogers

Book ID: 102822349
Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 577 KB
Volume: 4
Category: Article
ISSN: 0014-2980
DOI: 10.1002/eji.1830041007

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✦ Synopsis

Abstract

HL‐A‐active protein fragments have been isolated from lymphoid sources (tonsillar tissue, cells from lymphoblastoid cell lines (LCL cells)) and from platelets. The yields and specific activities of products obtained by enzymic chaotropic treatment indicate the superiority of 3 M KCl extraction of platelets. Individual HL‐A antigenic specificities were further distinguished in fractions separated by polyacrylamide gel electrophoresis. Antigens were separated between the LA and FOUR loci, and within the LA locus. Purified monospecific HL‐A antigenic preparations were conjugated with aminoethyl‐Sepharose‐4B, and used to remove corresponding specificities from alloantisera.

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