Isolation and purification of large quan
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Gijsbert Heuff; Arjan A. De Van Loosdrecht; Michiel G. H. Betjes; Robert H. J. B
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Article
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1995
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John Wiley and Sons
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English
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A new rapid method is described for the isolation and purification of functional active human Kupffer cells without the need of in situ perfusion techniques. Liver wedge biopsies (3 to 5 g), obtained after laparotomy, were incubated with pronase under continuous pH registration. Human Kupffer cells