We measured the concentration of hepatocyte growth factor after hepatectomy or CCl 4 -induced hepatic injury. HGF is a heterodimeric polypeptide composed of a 69-kd a-subunit (HGF) in bile obtained from patients after hepatectomy. The HGF concentrations in the bile samples were quantified using and a 34-kd b-subunit, both which are held together by disulfide bonds. 6,7 A high homology (91%) exists in the sequence an enzyme-linked immunosorbent assay (ELISA). By immunoblotting, using a monoclonal antibody raised against the of amino acids between rat and human HGF. It has been shown that sera from patients with fulminant hepatic failure HGF a-subunit, the bile HGF, which was purified on a Heparin-Sepharose column, showed a band of the same size as can stimulate DNA synthesis in adult rat hepatocytes in primary culture, 9 and that this may be due to the presence of the recombinant HGF a-subunit (69 kd). Bile samples were obtained from 24 patients with biliary tract disease before HGF. In humans, serum HGF concentrations increase in patients with hepatitis or in patients who have undergone and after hepatectomy by means of biliary drainage. Before surgery, the bile HGF concentrations were minimal (0.8 { hepatectomy 11-14 in response to liver regeneration, and the concentration of HGF increases in the peritoneal fluid after 0.1 ng/mL); however, after hepatectomy on postoperative day 1 in patients without posthepatectomy liver failure (20 of 24), hepatectomy. 15 Furthermore, injected, 125 I-labeled HGF is excreted into the bile of partial hepatectomy rats. Umeshita they increased severalfold (4.1 { 0.4 ng/mL, P รต .05). The patients with posthepatectomy liver failure (4 of 24) showed et al. have shown that the bile interleukin-6 concentration increases after liver transplantation. These results suggest that no significant increase in bile HGF after hepatectomy (less than 2 ng/mL on postoperative day 1). The volume of the HGF produced in the liver or in other organs can be excreted from the liver into the bile, together with other protein factors.
remnant liver correlated positively with the bile HGF concentration. The bile HGF concentration on postoperative day 1
In the present study, we measured the HGF concentration in bile samples obtained from hepatectomized patients whose exhibited a significant negative correlation with the maximum concentration of serum total bilirubin after hepatectomy. The remnant livers may have started to regenerate. In this study, we performed various types of hepatic segmentectomy with concentration of bile HGF was generally higher than that in serum (2.1-fold). Thus, the bile HGF concentration after caudate lobectomy for hilar cholangiocarcinoma or advanced gallbladder carcinoma involving the hepatic hilus, [19][20][22] which hepatectomy may be useful for the early assessment of posthepatectomy liver function. (HEPATOLOGY 1997;26:1092-also requires resection and reconstruction of the biliary system. Because most such patients suffer from obstructive jaun-1099.)
dice, percutaneous transhepatic biliary drainage is routinely performed to reduce the operative risk, 23 and attain a precise Hepatocyte growth factor (HGF), first identified in the sepreoperative diagnosis. 24,25 We, therefore, can obtain bile rum of rats that had undergone partial hepatectomy, is a posamples from all patients before and following surgery. Using tent mitogen for primary cultured hepatocytes. 1 It was first an enzyme-linked immunosorbent assay (ELISA), a signifipurified from rat platelets 2 and is considered to be a hepatocant amount of HGF was detected in the bile of patients after trophic factor that triggers compensatory liver regeneration hepatectomy. The increase in HGF was analyzed with respect to the prognosis of each patient, the serum HGF concentration, and other indicators, such as the total bilirubin concen-Abbreviations: HGF, hepatocyte growth factor; ELISA, enzyme-linked immunosortration in the serum, all of which are good markers of postopbent assay.