Levels of the human hepatocyte growth factor in serum of patients with various liver diseases determined by an enzyme-linked immunosorbent assay

✍ Scribed by Hirohito Tsubouchi; Yoshiyuki Niitani; Shuichi Hirono; Hiroyuki Nakayama; Eiichi Gohda; Naokatu Arakaki; Osami Sakiyama; Kozo Takahashi; Masayoshi Kimoto; Shuichi Kawakami; Minami Setoguchi; Tetsuya Tachikawa; Sadahito Shin; Terukatsu Arima; Yasushi Daikuhara

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 609 KB
• Volume: 13
• Category: Article
• ISSN: 0270-9139
• DOI: 10.1002/hep.1840130102

✦ Synopsis

We have found a hepatotrophic factor in plasma or sera of patients with fulminant hepatic failure and have purified human hepatocyte growth factor from plasma of these patients. In this study we developed an enzyme-linked immunosorbent assay with high specificity and sensitivity for human hepatocyte growth factor in human serum. This assay for serum human hepatocyte growth factor is a sandwich method consisting of three steps. The standard curve for human hepatocyte growth factor appeared to be linear in the range of 0.20 to 12.50 ng purified human hepatocyte growth factorlml (2.35 to 147 pmolb). The assay took about 4 hr. Serum human hepatocyte growth factor values in patients with fulminant hepatic failure measured by enzyme-linked immunosorbent assay showed a strong positive correlation with that by bioassay using rat hepatocytes in primary culture. The mean value of serum human hepatocyte growth factor for 30 normal subjects was 0.24 f 0.12 (S.D.) ng/ml; that for 23 patients with fulminant hepatic failure was 8.06 * 1.76 (S.E.M.) ng/ml-> 30 times greater than the mean value for normal subjects. Serum human hepatocyte growth factor levels in patients with acute hepatitis, chronic hepatitis and cirrhosis were found to be slightly higher than those in normal subjects, but only the increase in serum human hepatocyte growth factor of acute hepatitis patients was statistically significant. The enzyme-linked immunosorbent assay for serum human hepatocyte growth factor should prove useful