Human bone marrow colony growth in agar-gel

✍ Scribed by Beverley L. Pike; William A. Robinson

• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: English
• Weight: 609 KB
• Volume: 76
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040760111

✦ Synopsis

Abstract

A technique for growing human bone marrow cell colonies in agar‐gel medium is described. “Feeder layers” containing 1 × 10^6^ normal human peripheral white blood cells are used as the stimulus for colony growth. Human bone marrow aspirates are collected in heparinized syringes and plated as 2 × 10^5^ cells on “feeder layers.” Normal human bone marrow yields 32–102 colonies per 2 × 10^5^ cells plated. Colonies are almost exclusively granulocytic. Growth rate of colonies is slower than with mouse bone marrow but colonies reach a comparable size (500–1500 cells) at days 12–16.