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Human BLCAP transcript: new editing events in normal and cancerous tissues

✍ Scribed by Federica Galeano; Anne Leroy; Claudia Rossetti; Irina Gromova; Philippe Gautier; Liam P. Keegan; Luca Massimi; Concezio Di Rocco; Mary A. O'Connell; Angela Gallo

Publisher
John Wiley and Sons
Year
2009
Tongue
French
Weight
609 KB
Volume
127
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Bladder cancer‐associated protein (BLCAP) is a highly conserved protein among species, and it is considered a novel candidate tumor suppressor gene originally identified from human bladder carcinoma. However, little is known about the regulation or the function of this protein. Here, we show that the human BLCAP transcript undergoes multiple A‐to‐I editing events. Some of the new editing events alter the highly conserved amino terminus of the protein creating alternative protein isoforms by changing the genetically coded amino acids. We found that both ADAR1 and ADAR2‐editing enzymes cooperate to edit this transcript and that different tissues displayed distinctive ratios of edited and unedited BLCAP transcripts. Moreover, we observed a general decrease in BLCAP‐editing level in astrocytomas, bladder cancer and colorectal cancer when compared with the related normal tissues. The newly identified editing events, found to be downregulated in cancers, could be useful for future studies as a diagnostic tool to distinguish malignancies or epigenetic changes in different tumors.

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