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Human B lymphocytes in giemsa stained preparations

โœ Scribed by C.R. Taylor; I.L. Gordon; A. Rembaum; R. Russell; J. Parker; R.L. O'Brien; R.J. Lukes


Book ID
118897145
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
579 KB
Volume
17
Category
Article
ISSN
0022-1759

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The spindle apparatus of human mitoses can be preferentially stained with Giemsa if the cells are fixed with methanol--acetic acid and treated for about 15 min with 0.1 X SSC at 92 degrees C and for 18 h with 2 X SSC at 60 degrees C.