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HPLC quantification of dye flavonoids in Reseda luteola L. from Portugal

✍ Scribed by Cristina Moiteiro; Helena Gaspar; Ana I. Rodrigues; João F. Lopes; Valdemar Carnide


Book ID
102924701
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
501 KB
Volume
31
Category
Article
ISSN
1615-9306

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✦ Synopsis


Abstract

A HPLC method was developed for the simultaneous identification of Reseda luteola L. (weld) flavonoids and quantification of the main compounds responsible for the yellow color. This method was applied to a large number of wild Portuguese weld to evaluate its potential application as dyestuff for textile factories, as a substitute for the synthetic dyes currently used. Portuguese weld dyestuff content ranged between 1.04 and 5.87%, corresponding to a wide variation of the flavonoids amount (1.39–9.04%). Luteolin 4′‐O‐glucoside was found for the first time in R. luteola, but kaempferol, isorhamnetin, and their glycosides were not detected in the Portuguese specimens.


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A HPLC-diode array detector (DAD) methodology was developed to allow the simultaneous identification and quantification of Reseda luteola L. (weld) dye flavonoids, luteolin, apigenin, luteolin 7-O-glucoside, apigenin 7-O-glucoside, luteolin 3 0 ,7-O-diglucoside and luteolin 4 0 -O-glucoside. The met