HPLC of Sertraline and Norsertraline in Plasma or Serum

✍ Scribed by Jignasha Patel; E. P. Spencer; R. J. Flanagan

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 329 KB
• Volume: 10
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199611)10:6<351::aid-bmc620>3.0.co;2-o

✦ Synopsis

A simple method for the measurement of sertraline and norsertraline in plasma or serum suitable for use in single-dose pharmacokinetic studies has been developed. Internal standard solution, aqueous fenethazine (10 mg/L) (20 pL), and 'his buffer (2 mom), pH 10.6) (100 pL) were added to plasma (200 pL). Sertraline, norsertraline and the internal standard were extracted into methyl terr-butyl ether (200 pL) by mixing (30 s) and centrifugation (11, OOO r.p.m., 4 min). A portion (100 pL) of the extract was injected onto a Spherisorb SSSCX HPLC column (1SOx 4.6 mm id.) which was eluted with methanokwater (19+ 1) containing ammonium perchlorate (40 mmoVL), final pH 7.0. Detection was by UV monitoring (215 nm). The concentration of each analyte in each sample was calculated from the calibration graph (peak-height ratio of analyte to that of the internal standard against analyte concentration) obtained after analysis of plasma samples containing known amounts of sertraline and norsertraline. The limit of accurate measurement of the assay was 10 p&) sertraline and 20 pg/L)norsertraline. Sertraline [( lS,4S)-4-(3,4-dichlorophenyl)-1.2,3,4-tetrahydro-N-methyl-1-naphthylamine, Fig. 1) is a 1 -amino-