HPLC of fatty acid esters of mono- and polyhydric alcohols. Part 2: Preparative separation

✍ Scribed by Henke, H. ;Dietrich, W.

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 541 KB
• Volume: 3
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240030604

✦ Synopsis

Abstract

This paper demonstrates how simply and rapidly fatty acid esters of mono‐ and polyhydric alcohols can be separated quantitatively in preparative quantities on Lobar® RP‐8 packed columns. After the separation of unknown mixtures, the isolated esters are identified from spectroscopic data (IR/NMR) and, after saponification of the ester components (fatty acids and alcohols), from the retention times of gas and high‐pressure liquid chromatographic separations. Thus, in particular, sparingly volatile or nonvolatile partial esters of polyhydric alcohols, as well as the long‐chained full esters, can be determined qualitatively and quantitatively.

The following fatty acid esters of mono‐ and polyhydric alcohols have been separated: the i‐propylesters of the laurinic and myristinic acids, the i‐butyl‐, i‐octyl‐ and i‐octadecyl‐esters of the palmitinic and stearic acids, the mono‐ and di‐fatty acid esters of the 1,3‐bis‐(2‐hydroxyethyl)‐5,5‐dimethylhydantoins, the mono‐, di‐ and tri‐esters of the trimethylalpropane and the full esters of the pentaerythrite.