HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure

✍ Scribed by Laura Mercolini; Maria Addolorata Saracino; Francesca Bugamelli; Anna Ferranti; Marco Malaguti; Silvana Hrelia; Maria Augusta Raggi

• Book ID: 102926902
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 770 KB
• Volume: 31
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200700458

✦ Synopsis

Abstract

An original analytical method has been developed for the determination of the antioxidants __trans‐__resveratrol (__t‐__RSV) and __cis‐__resveratrol (__c‐__RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mm×4.6 mm id, 5 μm) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at λ = 386 nm while exciting at λ = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03–5.00 ng/mL for MLT, 3–500 ng/mL for __t‐__RSV and 1–150 ng/mL for __c‐__RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for __t‐__RSV and 0.3 ng/mL for __c‐__RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated.