HPLC-chip-mass spectrometry for protein signature identifications

✍ Scribed by Julie Hardouin; Raymonde Joubert-Caron; Michel Caron

• Book ID: 102440716
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 381 KB
• Volume: 30
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600444

✦ Synopsis

Abstract

This work investigates the use of an HPLC‐chip microfluidic device interfaced to an IT mass spectrometer to search for biomarker signatures. To that end, the identification of autoantigens is chosen as a model. It not only constitutes a proof of concept model but also the growing interest in autoantibodies and autoantigens as new markers of diseases provides a practical application at the same time. The peptides are separated by the HPLC‐chip system allowing suitable resolution and reproducibility. The determination of two parameters that characterize a peptide sequence during LC‐MS/MS analyses, retention time (RT) and m/z ratio, improves the identification of a number of peptides derived from protein digests. These findings illustrate that accurate RT measurement obtained in a microfluidic device is useful to obtain mass/retention time (MRT) pairs for a given peptide, which can contribute to the definition of biomarker signatures.