HPLC and 13C-NMR study of carboxymethyl-β-(1 → 6)-D-gluco-β-(1 → 3)-D-glucan derived from saccharomyces cerevisiae

✍ Scribed by Ladislav S̆oltés; Juraj Alföldi; Jozef S̆andula

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 475 KB
• Volume: 48
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.1993.070480801

✦ Synopsis

Sodium salt of carboxymethyl-P-( 1 + 6 ) -D-gluco-P-( 1 --t 3) -D-glucan ( CMG-Na) was prepared from 0-D-glucan isolated from baker's yeast ( Saccharomyces cerevisiae) . Three samples, Fractions I, 11, and 111, were further separated from the crude CMG-Na derivative. For the physicochemical characterization of the separated fractions, the methods of highperformance liquid chromatography (HPLC ) in the size-exclusion mode and carbon-13 nuclear magnetic resonance ( 13C-NMR) spectroscopy were applied. The HPLC method revealed that the molecular weights, M,, M,, and M , averages, of Fraction I1 were 9.71 X lo4, 2.27 X lo5, and 3.59 X lo5 Da, respectively, whereas those of Fraction 111 were 1.52 X lo4, 2.13 X lo4, and 3.57 X lo4 Da, respectively. The 13C-NMR spectra of Fraction 11 showed a ratio of 3 : 1 for 6-( 1 -3)/P-( 1 -6 ) , whereas for Fraction 111, the content of 0-( 1 -3 ) unit.s was smaller.