HPLC (A Practical User's Guide) || Application Logics: Separations Overview

✍ Scribed by McMaster, Marvin C.

• Publisher: John Wiley & Sons, Inc.
• Year: 2006
• Tongue: English
• Weight: 129 KB
• Edition: 2
• Category: Article
• ISBN: 0471754013
• DOI: 10.1002/9780470079096.ch13

✦ Synopsis

At this point, I am going to try something a little different. Most HPLC texts include a series of figures showing you a separation, including the conditions, for various classes of compounds. I prefer to give you the tools to predict new separations. First, to give you an approximate set of conditions for making almost any type of separation, and, second, indicate why a particular column, mobile phase, and detector (or wavelength) was chosen for this separationthe logics of the separation.

To address the first objective, I've included my separation guide (Appendix A), designed as a quick reference to conditions that could be adapted to separate compounds similar in polarity, in size, in charge, or in absorption. Where possible, isocratic runs were chosen, rather than gradients.To handle the second objective, we will go through the various classes of materials exploring the chemical and physical differences that dictate certain HPLC conditions.