HPLC (A Practical User's Guide) || Appendix A: Personal Separations Guide

✍ Scribed by McMaster, Marvin C.

• Publisher: John Wiley & Sons, Inc.
• Year: 2006
• Tongue: English
• Weight: 93 KB
• Edition: 2
• Category: Article
• ISBN: 0471754013
• DOI: 10.1002/9780470079096.app1

✦ Synopsis

have enclosed seven items as parts of the Appendix. The first, a separations guide, points out starting points for chromatographic separations and also suggests trends in usage of columns, mobile phase, and detectors.

The second item is a list of frequently asked questions (FAQs) that I have encountered while serving as an in-house trouble-shooting resource for customers and as an instructor for extension courses. I taught 30 two-day HPLC course in seven states before I began teaching at the University of Missouri-St. Louis. Many of these answers arose as responses to problems raised by students in my classes.

In the third appendix, I have added tables of solvents and volatile buffers important for use in LC/MS. Nonvolatile buffers cause problems when you remove solvent and ionize effluent for injection into the mass spectrometer and need to be avoided when this detector has been selected.

The fourth item is a glossary of HPLC terms. I have tried to include much of the terminology and buzzwords used in the field.

The fifth part is a trouble-shooting quick reference. It is not intended to replace the systematic trouble-shooting discussion in Chapter 10. When things go wrong, however, you may find it helpful. I have arranged it in the way things flow through the system: from pumps to the integrator or data acquisition computer.

The sixth item is a series of three HPLC laboratory experiments. The first familiarizes the student with getting a system up and running and calibrating a column with standards. The second experiment shows how to clean a column and pacify a system. The last is a first, quick look at methods development.