HPLC (A Practical User's Guide) || Appendix E: HPLC Troubleshooting Quick Reference

✍ Scribed by McMaster, Marvin C.

• Publisher: John Wiley & Sons, Inc.
• Year: 2006
• Tongue: English
• Weight: 96 KB
• Edition: 2
• Category: Article
• ISBN: 0471754013
• DOI: 10.1002/9780470079096.app5

✦ Synopsis

This section is designed to assist in troubleshooting system problems. It is not a systematic approach as described in Chapter 10, which always yields better results. Keeping this in mind, I have listed a series of commonly seen problems, possible causes, and suggested treatments. Problem 1: No power. (Display does not light up on module or system.) Cause a: Not plugged in. Treatment: Check the plug at the socket and at the module. It may have worked loose or been unplugged accidentally. Cause b: No fuse, blown fuse, or incorrect fuse.

Treatment: On a new system, make sure fuse(s) were installed. If variable voltage, make sure the selection card is correctly positioned. New systems are often shipped with fuses in a bag. If fuse is broken, replace it and contact service. Cause c: Not switched on.

Treatment: Turn on the switch (from the front, try the upper right-hand side of the back plate). Someone may have been helping you to conserve energy. I always set my system up on a common surge protector with an on/off switch. That way I can turn everything on with one switch and protect against line surges and ground loops at the same time. Problem 2: Leaking frits, puddles on desk top, fountains of solvent.

Cause a: Compression fittings not tight enough.

Treatment: Tighten leaking fitting another 1/4 turn or until leak stops. (Leaks will be at the back of the fitting around the tubing.)