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How to demonstrate specificity of antibody binding to synthetic peptides?

โœ Scribed by Wim van 't Hof; Florine J. van Milligen; Peter C. Driedijk; Marjan van den Berg; Rob C. Aalberse


Book ID
118902737
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
249 KB
Volume
161
Category
Article
ISSN
0022-1759

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Monoclonal antibodies (MAbs) were prepared to a synthetic peptide (PI : I I94 40 amino acids) in domain I of the carcinoembryonic antigen (CEA) molecule. The majority of the amino acids in peptide P I show a hydrophilic character, and similar sequences are repeated in domains I, II and 111 of this m