Host-plasmid interactions in gram-negative bacteria harbouring broad-host range plasmids

✍ Scribed by E. Bernardez; P. Dhurjati

• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 426 KB
• Volume: 3
• Category: Article
• ISSN: 1615-7605
• DOI: 10.1007/bf00373478

✦ Synopsis

Host-plasmid interactions were studied for the broadhost range plasmid RK2 and its derivative pTJS26. To isolate host and plasmid contributions to the host-plasmid interaction, experiments were performed with the same plasmid in three different gram-negative hosts. The three hosts were: Pseudomonas putida, Pseudomonas aeruginosa and Escherichia coli. Separate experiments were performed for both host and recombinant cells to identify their growth characteristics. Cells were grown in different media to investigate the effect of growth rate on plasmid stability, and at two different temperatures (30~ and 37~ to investigate the effect of plasmid content on growth dynamics. The comparison of the results obtained with plasmids RK2 and pTJS26 was used to analyze the effect of mutations in the replication region on growth dynamics and plasmid stability.