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Horseradish peroxidase extraction and purification by aqueous two-phase partition

✍ Scribed by Mar’ia V. Miranda; H’ector m. Fern’andez Lahore; Osvaldo Cascone

Book ID
112894169
Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
380 KB
Volume
53
Category
Article
ISSN
0273-2289

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Partition behaviour of horseradish perox
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Partition of purified horseradish peroxidase isoenzymes in aqueous two-phase systems was not affected by pH changes, but tie-line length and NaCl addition greatly increased the partition coefficient of ail three isoenzymes, the former having more influence than the latter. In all systems, K were hig

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Performance of an aqueous two-phase-based countercurrent chromatographic system for horseradish peroxidase purification
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## Abstract Countercurrent chromatography (CCC) purification of horseradish peroxidase (HRP) from __Armoracia rusticana__ root extracts was achieved by employing polymer‐phosphate aqueous two‐phase systems (ATPS). By using preparative columns at 1000 rpm, a 25–30% retention of the top phase of an A