The effect of amino acid depletion or supplementation and the effect of glucagon and insulin on the amino acid transport mediated by s stem A were acid (AIB) or N-methyl 2-amino [l-'4C]isobutyric acid (MeAIB) in rat hepatocytes, freshly isolated at different stages of pre-and postnatal development.
Hormonal regulation of gluconeogenesis in isolated rat hepatocytes: An undergraduate experiment
β Scribed by David A. hume; Maurice J. weidemann
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 199 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0307-4412
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Amino acid transport was studied in primary cultures of parenchymal cells isolated from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in a serum-free culture medium. These cells carried out gluconeogenesis from three carbon precursors (alanine, pyruvate, and lact
## Abstract The effects of insulin, glucagon or Dexamethasone (DEX) and of glucagon with insulin or DEX were examined on the uptake of 2βamino [1β^14^C]isobutyric acid (AIB) and NβMethylβ2βamino [1β^14^C]isobutyric acid (NMe AIB) in monolayer cultures of rat hepatocytes. Insulin and glucagon stimul