## Abstract Bone cells were isolated by collagenaseβhyaluronidase digestion of 19β20 dayβold fetal rat calvaria. It is shown that contamination from calcified matrix can give erroneously high values for total cell calcium and can mask intracellular calcium exchange. Filtration of the cell suspensi
Hormonal and nonhormonal desensitization in isolated bone cells
β Scribed by William A. Peck; Gail Kohler
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 886 KB
- Volume
- 32
- Category
- Article
- ISSN
- 1432-0827
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## Abstract Calcium transport was studied in bone cells isolated from fetal rat calvaria. ^45^Ca uptake experiments revealed an active component of calcium exchange. Calcium uptake was inhibited by iodoacetamide, DNP, CCCP and oligomycin and appeared to be dependent on medium phosphate concentratio