Calcium transport in isolated bone cells II. Calcium transport studies

✍ Scribed by Rosemary Dziak; John S. Brand

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 912 KB
• Volume: 84
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040840110

✦ Synopsis

Abstract

Calcium transport was studied in bone cells isolated from fetal rat calvaria. ^45^Ca uptake experiments revealed an active component of calcium exchange. Calcium uptake was inhibited by iodoacetamide, DNP, CCCP and oligomycin and appeared to be dependent on medium phosphate concentration. Initial influx values exhibited saturation kinetics from 0.6 mM to 1.5 mM extracellular calcium.

Efflux of ^45^Ca from loaded cells increased in the presence of iodoacetamide, DNP and CCCP. Incubation of the cells af 4° C inhibited both influx and efflux of calcium.

Parathyroid hormone had no consistent effect on calcium uptake although characteristic increases in cyclic AMP levels were seen with the hormone. Calcitonin appeared to cause a transient increase in calcium uptake.