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Homogeneous Noncompetitive Immunoassay for 17β-Estradiol Based on Fluorescence Resonance Energy Transfer

✍ Scribed by Kokko, Tiina; Kokko, Leena; Lövgren, Timo; Soukka, Tero


Book ID
127205404
Publisher
American Chemical Society
Year
2007
Tongue
English
Weight
200 KB
Volume
79
Category
Article
ISSN
0003-2700

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We describe a noncompetitive homogeneous bioluminescent immunoassay based on the antigen-dependent reassociation of antibody variable domains (open sandwich bioluminescent immunoassay, OS-BLIA). The reassociation of two chimeric proteins, an antibody heavy-chain fragment (V(H))-Renilla luciferase (R